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We calculated these sharing profiles for each ancient and modern individual independently, with the modern populations used and hence K varying across datasets, and performed jack-knifing across chromosomes to assess uncertainty. See SI Appendixsection 16 for further details. We first treated our 38 usable ancient samples as a single population. We then determined the likelihood that each sample in turn belonged to this single population by estimating the probability of observing a particular pair of haplotypes, given the population haplotype frequencies.
F IS and F ST were estimated across the individual 1-kb loci for groups of ancient samples and modern populations using the estimators of Nei 44 and Bhatia et al.